細(xì)胞培養(yǎng)物由支原體污染保持在細(xì)胞培養(yǎng)物中的一個主要問題。支原體能產(chǎn)生無限種在他們感染的培養(yǎng)影響。本文締一生物/締一生物為您分析細(xì)胞培養(yǎng)支原體污染：發(fā)生率，來源，效果，檢測，消除，預(yù)防。

　　這些微生物是在細(xì)胞培養(yǎng)中常用的多數(shù)抗生素抗性。在這里，我們提供關(guān)于當(dāng)前知識的一簡要的概述：

（1）發(fā)生率和支原體污染的在細(xì)胞培養(yǎng)物來源，支原體物種在細(xì)胞培養(yǎng)物zui常檢測到的，和支原體的上的功能和被感染細(xì)胞培養(yǎng)物的影響的活動;

（2）可用于檢測特別強調(diào)zui可靠的檢測方法支原體的各種技術(shù);

（3）可用于消除支原體突出抗生素治療的各種方法;

（4）推薦用于支原體污染的檢測，消除和預(yù)防程序和工作協(xié)議。準(zhǔn)確，靈敏和可靠的檢測方法的可用性和健壯和成功的消除方法的應(yīng)用提供用于克服在細(xì)胞培養(yǎng)物的支原體污染問題的有力手段。

英文原文：

The contamination of cell cultures by mycoplasmas remains a major problem in cell culture.Mycoplasmas can produce a virtually unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed in cell cultures.

Here we provide a concise overview of the current knowledge on:

(1) the incidence and sources of mycoplasma contamination in cell cultures, the mycoplasma species most commonly detected in cell cultures, and the effects of mycoplasmas on the function and activities of infected cell cultures;

(2) the various techniques available for the detection of mycoplasmas with particular emphasis on the most reliable detection methods;

(3) the various methods available for the elimination of mycoplasmas highlighting antibiotic treatment;

(4) the recommended procedures and working protocols for the detection, elimination and prevention of mycoplasma contamination. The availability of accurate, sensitive and reliable detection methods and the application of robust and successful elimination methods provide powerful means for overcoming the problem of mycoplasma contamination in cell cultures.

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